AGTC Genomics

Shotgun Metagenomic Sequencing

Shotgun metagenomic sequencing provides information on the total genomic DNA from all organisms in a sample, avoiding the need for isolation and cultivation of microorganisms or amplification of target regions. This is crucial because it is believed that nearly 99% of all microorganisms cannot be cultivated in the laboratory. Differently from the targeted approach used in the 16S/18S/ITS amplicon sequencing, shotgun metagenomic sequencing uses next-generation sequencing (NGS) technology to provide not only information on the taxonomic annotations of each organism but also the functional profiling, gene prediction and microbial interaction of the whole community.

Overview

Shotgun metagenomic sequencing provides information on the total genomic DNA from all organisms in a sample, avoiding the need for isolation and cultivation of microorganisms or amplification of target regions. This is crucial because it is believed that nearly 99% of all microorganisms cannot be cultivated in the laboratory. Differently from the targeted approach used in the 16S/18S/ITS amplicon sequencing, shotgun metagenomic sequencing uses next-generation sequencing (NGS) technology to provide not only information on the taxonomic annotations of each organism but also the functional profiling, gene prediction and microbial interaction of the whole community.
With extensive experience and well-developed bioinformatics pipelines, we can help you determine the genomes, functions, and variations of microorganisms from diverse samples. Our shotgun metagenomic sequencing service delivers high-quality data, publication-ready results, and personalized analysis to meet different research objectives. We offers comprehensive metagenomics solutions from the study of community structure and species classification to system evolution, gene function, and metabolic network of environmental microorganisms.

What’s Included

  • Sample Receipt and Initial QC
  • Library Construction and QC
  • Sequencing and Data Delivery
  • Bioinformatics Analysis

Input Requirements

  • ≥ 200 ng genomic DNA (≥ 10 ng/μL)

Data Deliverable

  • ≥ 25 million read pairs per sample for species with reference genome
  • Filtering host genome sequences
  • Assembly
  • Species annotation
  • Gene prediction
  • Gene annotation (KEGG, eggNOG, CAZy, ARDB databases)
  • Species/Gene/Function abundance statistics and cluster analysis
  • Comparative analysis (among samples)
Kyoto Encyclopedia of Genes and Genomes (KEGG) Annotation and Linear Discriminant Analysis (LDA) & Cladogram