Whole Transcriptome Sequencing
Whole Transcriptome Sequencing allows characterization of all types of RNA transcripts (coding and non-coding RNAs) of a particular organism, irrespective of whether or not they are polyadenylated. Whole transcriptome sequencing is advocated for both differential expression analysis as well as discovery work.
Overview
Whole Transcriptome Sequencing allows characterization of all types of RNA transcripts (coding and non-coding RNAs) of a particular organism, irrespective of whether or not they are polyadenylated. Whole transcriptome sequencing is advocated for both differential expression analysis as well as discovery work.
Our Whole Transcriptome Sequencing service offers a range of solutions tailored to your study’s needs. Each product has been optimized to yield the highest quality, most reliable results for its given applications, from differential expression analysis to the discovery and identification of mutations and fusion transcripts, even in FFPE tissue.
Input Requirements
- 250ng of purified total RNA
- FFPE* tissue, fresh/frozen tissue, blood, or cell pellets that preferably yield >250ng of RNA (separate charge will be applied for extractions)
* Due to the inherent nature of samples derived from FFPE to contain degraded and crosslinked nucleic acids, all samples submitted are accepted “on risk” and subject to billing regardless of data quality.
What’s Included
- Sample Receipt and Initial QC
- Library Construction and QC
- Sequencing and Data Delivery
- Bioinformatics Analysis
Data Deliverable
- 50 Million or 100 Million reads aligned pairs
- Data accessed via secure online digital transfer
- Quantitative expression profiles
- Alternative splicing analysis
- Fusion gene analysis
- Time series analysis
- Gene ontology analysis
- Pathway enrichment analysis
- Hierarchical clustering analysis
- Protein-Protein Interaction (PPI) analysis