AGTC Genomics

microRNA (miRNA) Sequencing

  • Identification of miRNA biomarkers for diagnosis and prognosis of diseases.
  • Characterization of miRNA-based therapeutic targets.
  • Investigating the mechanisms drug respins or resistance.
  • Study of miRNA-mediated gene regulation in different biological systems.


microRNAs (miRNAs) are small, non-coding RNA molecules that play important roles in regulating gene expression at the post-transcriptional level. miRNA sequencing allows researchers to measure the abundance of miRNAs in a sample and to compare the miRNA expression levels between different samples.


  • ≥ 2 μg of total RNA or ≥ 10 ng of exosomal RNA
  • A260/280 = 1.8-2.0
  • A260/230 ≥ 1.8

What’s Included

  • Sample Receipt and Initial QC
  • Library Construction and QC
  • Sequencing and Data Delivery
  • Bioinformatics Analysis

Data Deliverable

  • Summary of length distribution
  • Identification & characterization of miRNAs
  • Classification & annotation of miRNAs
  • Quantification & differential expression analysis
  • Functional enrichment analysis

Serum Exosomal MicroRNA, miR-10b-5p, as a Potential Diagnostic Biomarker for Early-Stage Hepatocellular Carcinoma. J Clin Med 2020, 9(1).