Single Cell Sequencing
APPLICATIONS
- Single Cell Gene Expression: Profiling of cellular heterogeneity, novel targets, and biomarker in each cell from a heterogeneous cell population.
- Single Cell Immune Profiling: Analyze full-length, paired B-cell or T-cell receptors, antigen specificity, and gene expression, all from a single cell.
OVERVIEW
Single Cell Sequencing reveals the full complexity of cellular diversity compared to bulk sequencing. It is primarily employed to solve complications related to the study of unusual cell types, cell-lineage associations, and samples of heterogeneous nature. By doing in-depth single-cell sequencing, the cell functions of individual cells can be explored easily and efficiently.
INPUT REQUIREMENTS
- Single nuclei suspension
What’s Included
- Sample Receipt and Initial QC
- Library Construction and QC
- Sequencing and Data Delivery
- Bioinformatics Analysis
Data Deliverable
- 10x sequencing data processing and quality control
- Identification of highly variable genes (HVGs)
- Cell subpopulation identification
- Marker gene detection
- Enrichment analysis and annotation of marker genes
Pan-cancer single-cell landscape of tumor-infiltrating T cells. Science 2021, 374(6574):abe6474.